Team Physiology of Gonadotrope Axis (ERL U1133 INSERM)

Head: Joëlle Cohen-Tannoudji (PR P7)

Main techniques used

  • Organotypic cultures, primary cultures of pituitary and granulosa cells, cultures of eukaryotic cell lines
  • Western blot, immunohistochemistry, ELISA of gonadotropic hormones, GST-pull down
  • In situ hybridization, qPCR, cloning, site-directed mutagenesis, stable and transient transfections of eukaryotic cells, measurement of promoter activity, in vivo chromatin immunoprecipitation on cells or tissues (ChIP), electrophoretic mobility shift assay (EMSA), transcriptional analyses (microarrays, ChIP on Chip)
  • Cellular signalling on Plateau FlexStation 3